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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal TEV Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G/S), with cleavage occurring between Q and G or Q and S. The most commonly used sequence is ENLYFQG.
BP002226-PRO-585: Recombinant Tobacco Etch Virus Protease (TEV) Protein
Source: E. coli-derived.
Recombinant Tobacco Etch Virus Protease (TEV) Protein is a site-specific protease. The protease can be used for the removal of affinity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30 ?; however, the enzyme can be used at temperatures as low as 4 C. The rTEV contains His tag. The rTEV is purified by proprietary chromatographic techniques.
The specific activity of Recombinant Tobacco Etch Virus Protease (TEV) is calculated by its ability to 10,000 Units/1mg.
Purity: > 90.0% as determined by (a) RP-HPLC; (b) SDS-PAGE.
Formulation: The rTEV contains 0.50 M Tris-HCl pH 8.0, 10 mM DTT and 5 mM EDTA.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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