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Conditions of optimal TCEA1 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
TCEA1 is a member of the TFS-II family. TCEA1 binds to Pol II and operates to cleave the emerging transcript, thus unlocking the complex and permitting transcription to continue. TCEA1 is localized to the nucleus and holds three independently-folding domains that are essential for accurate binding to Pol II. The arresting sites in are able to trap a certain segment of elongating RNA polymerases that goes through, causing in a locked ternary complex. Cleavage of the emerging transcript by S-II enables the renewal of elongation from the new 3'-terminus.
BP2019-PRO-1098: Recombinant Human Transcription elongation factor A (SII) 1 (TCEA1) Protein
Source: E. coli-derived.
Recombinant Human Transcription elongation factor A (SII) 1 (TCEA1) Protein is a single polypeptide chain containing 325 amino acids (1-301) and having a molecular mass of 36.5 kDa. Recombinant Human Transcription elongation factor A (SII) 1 (TCEA1) Protein is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Purity: > 90.0% as determined by SDS-PAGE.
Formulation: The TCEA1 solution (1 mg/ml) contains 20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 50 mM NaCl and 20% glycerol.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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