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Conditions of optimal SUMO2 Protein performance should be determined experimentally by the investigator.
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Fax: 1-617-606-5019
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Introduction
Small ubiquitin-related modifier 2, also known as Ubiquitin-like protein SMT3B, SMT3 homolog 2, SMT3A, SMT3H2, is an Ubiquitin-like protein that belongs to the ubiquitin family with SUMO subfamily. It is a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. SUMO2 can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.
BP001123-C176: Recombinant Human Small ubiquitin-related modifier 2 (SUMO2) Protein
Source: E. coli-derived.
Recombinant Human Small ubiquitin-related modifier 2 (SUMO2) Protein is expressed with sequence (Met1-Gly93) of Human SUMO2.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).
Formulation: 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other SUMO2 Proteins:
Recombinant Human SUMO2 Protein
Recombinant Human SUMO2 Protein, GST Tag
SUMO2 Antibodies:
SUMO2/3 (Cleaved-Gly93) Polyclonal Antibody
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