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Conditions of optimal SHH Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
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Fax: 1-617-606-5019
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Or leave a message with a formal purchase
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Introduction
Recombinant Human Sonic Hedgehog is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. 3 mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Human Sonic Hedgehog is 99% homologous to the mouse gene. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene.
BP000078-CYT-676: Recombinant Human Sonic HedgeHog (SHH) Protein
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing 175 amino acids and having a molecular mass of 19,683 Dalton.
The ED50, determined by the dose-dependent induction of alkaline phosphatase production by C3H10T1/2 fibroblasts is 760 ug/ml, corresponding to a specific activity of 1315.8 IU/mg.
Purity: > 97% by SDS-PAGE and RP-HPLC.
Formulation: Lyophilized from a concentrated (1 ug/ml) solution in water containing 10 mM sodium Phosphate buffer pH-7.5.
Reconstitution: It is recommended to reconstitute the lyophilized Sonic HedgeHog with sterile water at a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other SHH Proteins:
Recombinant Mouse SHH Protein, His Tag
Recombinant Human SHH Protein, His Tag
Recombinant Mouse SHH Protein
Recombinant Human SHH Protein
Recombinant Human SHH Protein
SHH Antibody:
SHH Polyclonal Antibody
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