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Conditions of optimal recombinant Rhesus macaque CD4/LEU3 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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BP005134: Rhesus macaque CD4/LEU3 Protein with the C-His Tag
Source: The recombinant Rhesus macaque CD4/LEU3 Protein, (amino acids Lys26-Trp390) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Rhesus macaque CD4/LEU3 protein with the His tag at the C-terminus has a predicted MW of 41.5 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Rhesus macaque CD4 protein with the C-His tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Anti-CD4 Antibody protein with the C-hFc tag with the EC50 of 14.6 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Rhesus macaque CD4/LEU3 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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