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Home > Chemicals > Amino Acids and Derivatives > Recombinant Human Insulin (Cell Culture Grade)

Recombinant Human Insulin (Cell Culture Grade)

rDNA human insulin, biosynthetic human insulin (BHI)

Catalog No. Product Name Size List Price (US$) Quantity
BP006254 Recombinant Human Insulin (Cell Culture Grade) 100 mg 150.00
BP006254 Recombinant Human Insulin (Cell Culture Grade) 250 mg 250.00
BP006254 Recombinant Human Insulin (Cell Culture Grade) 500 mg 400.00
BP006254 Recombinant Human Insulin (Cell Culture Grade) 1 g 600.00
Description

BP006254: Recombinant Human Insulin (Cell Culture Grade)

Background

Insulin is the sole hormone in the human body that lowers blood glucose levels, while simultaneously promoting the synthesis of glycogen, fats, and proteins. Recombinant human insulin is produced through genetic engineering by constructing an E. coli expression system, followed by bioreactor fermentation and purification processes. Its amino acid sequence is identical to endogenously expressed human insulin, making it a high-purity, animal component-free bioactive protein.

Currently, industrial-grade recombinant human insulin is predominantly used as a cell culture medium additive for animal cell cultivation. Cell culture media supplemented with recombinant human insulin can mimic in vivo growth conditions more authentically, thereby enhancing cellular proliferation and stabilizing expression of target proteins.

CAS No.: 11061-68-0.
Molecular Weight: 5807.57.
Molecular Formula: C257H383N65O77S6.
Appearance: White or almost white crystalline powder.
Biological Activity: Approx. 20 units/mg.
Purity: 95%-105%.
Endotoxin Level: ≤10 EU/mg.
Typical Zinc content: ≤1.0%.
Loss on Drying: ≤10.0%.
Isoelectric Point: Approx. 5.30.
Solubility: The recombinant human insulin is soluble in 0.01M HCl (20 mg/mL), yielding a clear colorless solution.
Applications: Recombinant human insulin is frequently employed as a culture supplement in cell cultivation systems. Historically recognized as a critical factor for regulating growth and differentiation in most cell types in vitro, its working concentration typically ranges from 1–10 µg/mL (dynamically optimized for specific cell types). In serum-free culture protocols, insulin is often immobilized onto polystyrene culture dish surfaces.

Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
24 months from date of receipt if stored at -20°C to -70°C as supplied.

 

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