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Rat Polyclonal Antibody Development Services

Rat Polyclonal Antibody Production, Custom Rat Polyclonal Antibody
Catalog No. Product Name Size List Price (US$) Quantity
BS015A Standard Rat Polyclonal Antibody Development Service 1 package 1500.00
BS015B Standard Rat Polyclonal Antibody Development Service + Standard Peptide Synthesis + Peptide Conjugation 1 package 1800.00
BS015C Standard Rat Polyclonal Antibody Development Service + Standard Peptide Synthesis + Peptide Conjugation + Antigen Affinity Purification 1 package 2200.00
BS015D Standard Rat Polyclonal Antibody Development Service + DNA Subcloning + Bacterial Expression and Purification 1 package 3600.00
BS015E Standard Rat Polyclonal Antibody Development Service + DNA Subcloning + Bacterial Expression and Purification + Protein A Affinity Purification 1 package 3900.00
Description

The most comprehensive rat polyclonal antibody production services: custom production of rat polyclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; rat polyclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications.

BS015A: $1500 for a standard 50-day 5-rat immunization program.

You provide: Ten mg of peptide (purity > 85%, length > 12 amino acids) or 4 mg of soluble protein (concentration > 0.4 mg/ml, purity > 90%, molecular weight > 20 kDa) for immunization, and additional 5 mg for antigen affinity purification.

Deliverable:
* 10 ul of pre-immune serum;
* 2-4 ml of antisera/rat;
* Remaining antigens;
* ELISA results.

The highest quality standard in the field of mouse monoclonal antibody production services: guaranteed ELISA > 1:12 000 (antisera) and > 1: 20 000 (purified antibodies).

Clients have the choice for licensing antibodies to save cost and even make money.

The price will be adjusted for the following requirements:

a. Peptide (12-15 amino acids, >85%) design and synthesis +$250 for standard, +$450 for mono-phosphorylated, +$650 for di-phosphorylated, +$700 for mono-methylated (K or R site), +$650 for di-methylated (K or R site), +$700 for tri-methylated (K site), +$400 for acetylated;
b. Polyclonal antibody production service for modified peptide: +$1 300 for phospho-specific;
c. Synthesis of other antigens, +$variable;
d. Conjugation of antigens to protein carriers +$150;
e. Protein: DNA subcloning +$300, bacterial expression and purification +$1 800;
f. Additional boost immunization for higher ELISA titer of the antisera +$100/injection;
g. Additional animal +$300/rat, +$300/mouse;
h. Antibody purification: antigen affinity purification +$400, protein A/G affinity purification +$300, ammonium sulfate precipitation +$200;
i. Guaranteed applications: Western blot +$1 500, IHC +$2 000.

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