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Conditions of optimal PPP1CA Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
Serine/Threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) is crucial for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. The PPP1CA protein may have a significant role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.
BP002719-ENZ-165: Recombinant Human Protein Phosphatase 1, Catalytic Subunit Alpha (PPP1CA)
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing 350 amino acids (1-330 a.a) and having a molecular mass of 39.7 kDa. PPP1CA is fused to a 20 amino acid His-tag at N-terminus.
Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37?, pH7.5 using 10 mM of substrate.
Purity: > 80.0% as determined by SDS-PAGE.
Formulation: PPP1CA protein solution (0.25 mg/ml) containing 50 mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15 M NaCl, 1mM DTT, 0.1 mM PMSF and 1 mM MnCl2.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
PPP1CA Antibodies:
PPP1CA Monoclonal Antibody
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