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Conditions of optimal PPM1G Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
Protein Phosphatase 1G (PP1MG) is a cytoplasmic protein that belongs to the PP2C family. PPM1G is widely expressed; it is abundant in testis, skeletal muscle, and heart. PPM1G is a negative regulator of cell stress response pathways. PPM1G is responsible for the dephosphorylation of Pre-mRNA splicing factors, an important factor for the formation of functional spliceosome. PPM1G also plays a role in regulating cell cycle progression.
BP000781-ENZ-156: Recombinant Human Protein Phosphatase 1G 546 a.a. (PPM1G) Enzyme
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing 566 amino acids (1-546) and having a molecular mass of 61.4 kDa. The PPM1G is fused to a 20 amino acid His-Tag at N-terminus.
Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 25C, pH7.5 using 10mM of substrate.
Purity: > 85% by SDS-PAGE.
Formulation: The PPM1G solution (1 ug/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 0.1 M NaCl, 0.1 mM PMSF and 20% glycerol.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
Other PPM1G Enzymes:
Recombinant Human PPM1G Enzyme
Recombinant Human PPM1G Enzyme
PPM1G Antibodies:
PPM1G Monoclonal Antibody
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