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Conditions of optimal PPCS Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Phosphopantothenate-cysteine ligase (PPCS) catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is a vital universal pathway in prokaryotes and eukaryotes. PPCS, being one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine.
BP002784-ENZ-139: Recombinant Human Phosphopantothenoylcysteine Synthetase (PPCS) Enzyme
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing 331 amino acids (1-311 a.a.) and having a molecular mass of 36.1 kDa. PPCS is fused to a 20 amino acid His-tag at N-terminus.
Purity: > 95.0% as determined by SDS-PAGE.
Formulation: PPCS protein solution (1 mg/ml) containing 20 mM Tris-HCl buffer (pH8.0) and 10% glycerol.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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