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Conditions of optimal PPA1 Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
PPA1 is a member of the PPase family. PPA1 catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. Many biochemical pathways use the hydrolysis of PPi to two phosphate ions to make the reactions permanently irreversible. A prominent illustration of this phenomenon is the inorganic pyrophosphatase catalyzation in the hydrolysis reaction at the beginning of lipid degradation. Inorganic pyrophosphatase provides the motivation for the activation of fatty acids destined for oxidation by stimulating the rapid hydrolysis of pyrophosphate.
BP002718-ENZ-241: Recombinant Human Pyrophosphatase-1 (PPA1) Enzyme
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing 313 amino acids (1-289) and having a molecular mass of 35.2 kDa. PPA1 is fused to a 24 amino acid His-tag at N-terminus.
Purity: > 95% as determined by SDS-PAGE.
Formulation: The PPA1 solution (0.5 mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 200 mM NaCl, 2 mM DTT and 20% glycerol.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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