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Conditions of optimal POU5F1 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
POU5F1 is a homeodomain transcription factor of the POU family, expressed in embryonic stem (ES) cells and embryonic carcinoma (EC) cells. POU5F1 is significantly involved in the signaling pathway for maintaining self-renewal and pluripotency of ES cells. POU5F1 has 2 distinct DNA binding domains which independently bind half-sites of the canonical octamer motif. This flexibility allows POU5F1 to bind with distinct DNA motifs by forming heterodimers with other transcription factors or by forming homodimers in several conformations. Human POU5F1 contains a 75aa POU specific (POUS) domain and a 60aa POU-Homeo-(POUH) domain connected by a linker region. The Human POU5F1 specifically interacts with Octamer motif ATGCAAAT. In addition, 2 proline-rich domains in the N-terminal and C-terminal regions are vital for POU5F1 transactivation. POU5F1 regulates a number of target genes and has been shown to work jointly with other transcription factors including Sox2 as well as Nanog to sustain stem cell potency and self-renewal.
BP002391-PRO-088: Recombinant Human POU domain class 5 transcription factor 1 (POU5F1) Protein
Source: E. coli-derived.
Recombinant Human POU5F1 protein is a single, non-glycosylated, Polypeptide chain containing 360 amino acids and having a molecular mass of 38.6 kDa. The POU5F1 protein is fused to an N-terminal TAT (transcriptional activator protein) tag and purified by proprietary chromatographic techniques.
N-terminal sequence analysis: Met-Gly-Arg-Lys-Lys.
Purity: > 95.0% as determined by SEC-HPLC and by SDS-PAGE.
Formulation: POU5F1 was lyophilized after extensive dialysis against PBS.
Reconstitution: It is recommended to reconstitute the lyophilized POU5F1 in sterile PBS not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other POU5F1 Proteins:
Recombinant Human POU5F1, Polyarginine-Tag Protein
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