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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal POLL Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
DNA polymerase POLL is involved in base excision repair (BER). POLL is a low-fidelity polymerase that takes part in both spontaneous and DNA damage-induced mutagenesis. Furthermore, POLL takes part in the mutagenic bypass of T-T dimers. Pol lambda possesses terminal transferase and 5?deoxyribose-5-phosphate lyase activity which is synchronized by PCNA and replication protein A.
BP002724-ENZ-182: Recombinant Human Polymerase (DNA directed), Lambda (POLL) Enzyme
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing 320 amino acids (1-300) and having a molecular mass of 36.0 kDa. POLL is fused to a 20 amino acid His-tag at N-terminus.
Purity: > 90% as determined by SDS-PAGE.
Formulation: The POLL solution (0.5 mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 0.1 M NaCl and 30% glycerol.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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