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Conditions of optimal PGK2 Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
PGK2 is a testis-specific form of phosphoglycerate kinase. Originally presumed to be a pseudogene, the PGK2 protein is actually a functional phosphoglycerate kinase which catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. The PGK2 gene is intronless, results from retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis.
BP002753-PKA-017: Recombinant Human Phosphoglycerate Kinase 2 (PGK2) Enzyme
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing 437 amino acids (1-417 a.a.) and having a molecular mass of 46.9 kDa. PGK2 is fused to a 20 amino acid His-tag at N-terminus.
Purity: > 85.0% as determined by SDS-PAGE.
Formulation: PGK2 protein solution (0.5 mg/ml) containing 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 1 mM DTT and 0.1 M NaCl.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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