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Conditions of optimal NTHL1 Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Nth endonuclease III-like protein 1 (NTHL1) is one of the enzymes which act on a large number of oxidatively damaged DNA sites. NTHL1 has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. NTHL1 acts on a damaged strand, 5' from the damaged site and incises damaged DNA at cytosines, thymines and guanines.
BP002530-ENZ-172: Recombinant Human Nth Endonuclease III-Like 1 (NTHL1) Enzyme
Source: E. coli-derived.
A single, non-glycosylated polypeptide chain containing 332 amino acids (1-312 a.a.) and having a molecular mass of 36.6 kDa. NTHL1 is fused to a 20 amino acid His-tag at N-terminus.
Purity: > 80.0% as determined by SDS-PAGE.
Formulation: NTHL1 protein solution (0.25 mg/ml) containing 20 mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15 M NaCl and 1 mM DTT.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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