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Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal NTH Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
Nth has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme cuts impaired DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5' from the damaged site. Nth is vital for the restoration of both oxidative DNA damage and spontaneous mutagenic lesions.
BP002529-ENZ-132: Recombinant E.Coli Endonuclease-III (NTH) Enzyme
Source: E. coli-derived.
A single, non-glycosylated, polypeptide chain containing 231 amino acids (1-211a.a.) and having a molecular mass of 25.7 kDa.
Purity: > 85.0% as determined by SDS-PAGE.
Formulation: The NTH solution (0.5 mg/ml) contains 20 mM Tris-HCl buffer (pH8.0), 0.1 M NaCl, 1 mM DTT, 0.1 mM PMSF and 40% glycerol.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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