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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal MYLPF Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Myosin regulatory light chains, including MRCL3, MYLPF and MYL9, regulate contraction in smooth muscle and non-muscle cells via phosphorylation by MLCK (myosin light chain kinase). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the actin-activated myosin ATPase activity, thus regulating the contractile activity. MYLPF is vital for fast and slow skeletal muscle development.
BP002379-PRO-243: Recombinant Human Myosin Light chain, Phosphorylatable, Fast Skeletal Muscle (MYLPF) Protein
Source: E. coli-derived.
Recombinant Human MYLPF protein is a single, non-glycosylated polypeptide chain containing 189 amino acids (1-169 a.a) and having a molecular mass of 21.2 kDa. MYLPF protein is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Purity: > 85.0% as determined by SDS-PAGE.
Formulation: MYLPF protein solution (0.25 mg/ml) containing 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol and 100 mM NaCl.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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