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EK000132-EK0452: Mouse MIP-2 ELISA Kit
Range 15.6pg/ml-1000pg/ml
Sensitivity < 5pg/ml
Specificity: No detectable cross-reactivity with any other cytokine.
Application: For quantitative detection of mouse MIP-2 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: Four months at 4°C and eight months at -20°C.
Background
MIP is a member of the aquaporin family of membrane-bound water channels.1 MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains.2 Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens.3 The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells.4 The standard used in this kit is recombinant mouse MIP-2(A28-N100), consisting of 73 amino acids with the molecular mass of 8Kda
Principle
MIP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse MIP-2 specific-specific monoclonal antibodies were precoated onto 96-well plates. The mouse specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-2 amount of sample captured in plate.
Reference
1. Berry, V., Francis, P., Kaushal, S., Moore, A., Bhattacharya, S. Missense mutations in MIP underlie autosomal dominant 'polymorphic' and lamellar cataracts linked to 12q. Nature Genet. 25: 15-17, 2000.
2. Chrispeels MJ, Agre P (1994). "Aquaporins: water channel proteins of plant and animal cells". Trends Biochem. Sci. 19 (10): 421?25.
3. Pisano, M. M., Chepelinsky, A. B. Genomic cloning, complete nucleotide sequence, and structure of the human gene encoding the major intrinsic protein (MIP) of the lens. Genomics 11: 981-990, 1991.
4. Shiels, A., Bassnett, S. Mutations in the founder of the MIP gene family underlie cataract development in the mouse. Nature Genet. 12: 212-215, 1996.
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