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EK000121-EK0708: Mouse B7-1/CD80 ELISA Kit
Range 62.5pg/ml-4000pg/ml
Sensitivity < 10pg/ml
Specificity: No detectable cross-reactivity with any other cytokine.
Application: For quantitative detection of mouse B7-1/CD80 in sera, body fluids, tissue lysates or cell culture supernates. The significance of detection in sera, plasma, body fluids is undetermined.
Expiration: Four months at 4°C and eight months at -20°C.
Background
The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain[1].The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.[2] stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1 D37?245?which is composed of two single chains acids with the dipolymer.
Principle
B7-1/CD80 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse B7-1/CD80 specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse B7-1/CD80 amount of sample captured in plate.
Reference
[1]. Reeves, R. H., Patch, D., Sharpe, A. H., Borriello, F., Freeman, G. J., Edelhoff, S., Disteche, C. The costimulatory genes Cd80 and Cd86 are linked on mouse chromosome 16 and human chromosome 3. Mammalian Genome 8: 581-582, 1997.
[2].Selvakumar, A., Mohanraj, B. K., Eddy, R. L., Shows, T. B., White, P. C., Dupont, B. Genomic organization and chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7. Immunogenetics 36: 175-181, 1992..
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