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EK000120-EK0703: Mouse CD40/TNFRSF5 ELISA Kit
Range 15.6pg/ml-1000pg/ml
Sensitivity < 1pg/ml
Specificity: No detectable cross-reactivity with any other cytokine.
Application: For quantitative detection of mouse CD40/TNFRSF5 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: Four months at 4°C and eight months at -20°C.
Background
CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells.1The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2.2The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.3
Principle
CD40/TNFRSF5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse CD40/TNFRSF5 specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD40/TNFRSF5 amount of sample captured in plate.
Reference
1?Clark, E. A. CD40: a cytokine receptor in search of a ligand. Tissue Antigens 35: 33-36, 1990.
2?Lafage-Pochitaloff, M., Herman, P., Birg, F., Galizzi, J.-P., Simonetti, J., Mannoni, P., Banchereau, J. Localization of the human CD40 gene to chromosome 20, bands q12-q13.2. Leukemia 8: 1172-1175, 1994.
3?"Entrez Gene: CD40 CD40 molecule, TNF receptor superfamily member 5".
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