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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal MAPK1 Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
Mitogen-activated protein kinase 1 (MAPK1) is also known as "extracellular signal-regulated kinase 2" (ERK2). Two similar (85% sequence identity) protein kinases were originally called ERK1 and ERK2. They were found during a search for protein kinases that are rapidly phosphorylated after activation of cell surface tyrosine kinasessuch as the epidermal growth factor receptor. Phosphorylation of ERKs leads to the activation of their kinase activity.
The molecular events linking cell surface receptors to activation of ERKs are complex. It was found that RasGTP-binding proteins are involved in the activation of ERKs. Another protein kinase, Raf-1, was shown to phosphorylate a "MAPK kinase", thus qualifying as a "MAPK kinase kinase". The MAPK kinase was named "MAPK/ERK kinase" (MEK). Receptor-linked tyrosine kinases, Ras, Raf, MEK and MAPK could be fitted into a signaling cascade linking an extracellular signal to MAPK activation. Transgenic gene knockoutmice lacking MAPK1 have major defects in early development.
BP000663-PKA-214: Recombinant Human MAPK1, Active
Source: E. coli-derived.
A highly active form produced by phosphorylation of the purified ERK2/MAPK1 in vitro with MEK1 is a non-glycosylated polypeptide chain containing amino acids 2-480 and having a molecular mass of 44.6 kDa.
Specific Activity is 470.000 Units/mg.
Purity > 95%, by SDS-PAGE.
Formulation: MAPK1 is supplied containing 50 mM Tris-HCL, 150 mM NaCl, 1 mM DTT, 50% glycerol, pH 8.5.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
Other MAPK1 Enzymes:
Recombinant Human MAPK1, His Tag
Recombinant Human MAPK1
MAPK1 Antibodies:
MAPK1 Monoclonal Antibody
MAPK1/3 Polyclonal Antibody
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