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EK000031-EK0439: Human Leptin receptor ELISA Kit
Range 156pg/ml-10,000pg/ml
Sensitivity < 8 pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human Leptin receptor in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
Leptin receptor (or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus.1 Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family.2 Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene (LEPR) might contribute to obesity or obesity-related diseases.3 Leptin is also involved in the regulation of blood pressure through the leptin receptor.4
Principle
The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.
Reference
1. Tartaglia, L. A.; Dembski, M.; Weng, X.; Deng, N.; Culpepper, J.; Devos, R.; Richards, G. J.; Campfield, L. A.; Clark, F. T.; Deeds, J.; Muir, C.; Sanker, S.; Moriarty, A.; Moore, K. J.; Smutko, J. S.; Mays, G. G.; Woolf, E. A.; Monroe, C. A.; Tepper, R. I. Identification and expression cloning of a leptin receptor, OB-R. Cell 83: 1263-1271, 1995.
2. Clement, K.; Vaisse, C.; Lahlou, N.; Cabrol, S.; Pelloux, V.; Cassuto, D.; Gourmelen, M.; Dina, C.; Chambaz, J.; Lacorte, J.-M.; Basdevant, A.; Bougneres, P.; Lebouc, Y.; Froguel, P.; Guy-Grand, B. A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction. Nature 392: 398-401, 1998.
3. Park, K. S.; Shin, H. D.; Park, B. L.; Cheong, H. S.; Cho, Y. M.; Lee, H. K.; Lee, J.-Y.; Lee, J.-K.; Oh, B.; Kimm, K. Polymorphisms in the leptin receptor (LEPR)--putative association with obesity and T2DM. J. Hum. Genet. 51: 85-91, 2006.
4. Rosmond, R.; Chagnon, Y. C.; Holm, G.; Chagnon, M.; Perusse, L.; Lindell, K.; Carlsson, B.; Bouchard, C.; Bjorntorp, P. Hypertension in obesity and the leptin receptor gene locus. J. Clin. Endocr. Metab. 85: 3126-3131, 2000.
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