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Conditions of optimal KLOTHO Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
Klotho is a type-I membrane protein which is related to beta-glucosidases. Reduced production of the Klotho protein is detected in patients with chronic renal failure (CRF), which may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. In addition, mutations within the Klotho protein are linked with ageing and bone loss.
BP002589-ENZ-369: Recombinant Human Klotho Protein
Source: CHO (Chinese Hamster Ovarian Cells)-derived.
The Recombinant Human Klotho Protein is fused with a C-terminal 6xHis Tag. The Klotho His Tagged Fusion Protein is 59.5 kDa protein containing a total of 522 amino acid residues and purified by proprietary chromatographic techniques.
Purity: > 90% as determined by SDS-PAGE.
Applications: ELISA, Immunoassays, WB.
Formulation: Human Klotho recombinant was filtered (0.4 um) and lyophilized from 0.5 mg/ml in 0.08 M phosphate buffer and 0.1 M NaCl, pH 7.2.
Reconstitution: It is recommended to add to Klothodeionized water to prepare a working stock solution of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter Klothoby an appropriate sterile filter before using it in the cell culture.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
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