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IL-2 Protein

Interleukin-2, T-Cell Growth Factor, TCGF, Aldesleukin, IL2

Catalog No. Product Name Size List Price (US$) Quantity
BP000145-C013 Recombinant Human IL-2 Protein 50 ug 180.00
BP000145-C013 Recombinant Human IL-2 Protein 1 mg 864.00
BP000145-C013 Recombinant Human IL-2 Protein 10 ug 98.00
Description

Introduction
Recombinant Human interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-125 human interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the human IL-2 gene, and the resulting expression clone encodes a modified human IL-2. This recombinant form differs from native interleukin-2 in following ways: 1) it is not glycosylated; 2) the molecule has no N-terminal alanine; 3) the molecule has serine substituted for cysteine at amino acid position 125; 4) the aggregation state of molecule is likely to be different from that of native IL-2.

BP000145-C013: Recombinant Human Interleukin-2 (IL-2) Protein

Source: E. coli-derived.
Recombinant Human Interleukin-2 (IL-2) Protein is expressed with sequence (Pro22-Thr153) of Human IL-2.
N-terminal sequence analysis: Met-Pro-Thr-Ser-Ser-Ser-Thr-Lys-Lys-Thr-Gln-Leu- Gln-Leu-Glu.
Predicted molecular mass: 15,300 Daltons.
The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is less then 0.1 ug/ml, corresponding to a Specific Activity of 1.0 x 107 IU/ mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).
Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM HAc-NaAc, 150 mM NaCl, pH 4.0.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.

Other IL-2 Proteins:
Recombinant Rat IL-2 Protein
Recombinant Porcine IL-2 Protein
Recombinant Mouse IL-2 Protein, His Tag
Recombinant Mouse IL-2 Protein
Recombinant Human IL-2 Protein, His Tag
Recombinant Human IL-2 Protein, HEK
Recombinant Human IL-2 Protein
Recombinant Human IL-2 Protein

IL-2 Antibodies:
IL-2 Polyclonal Antibody
IL-2 Polyclonal Antibody
IL-2 Monoclonal Antibody
IL-2 Monoclonal Antibody
IL-2 Monoclonal Antibody

IL-2 ELISA Kits:
Rat IL-2 ELISA Kit
Mouse IL-2 ELISA Kit
Human IL-2 ELISA Kit

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