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Human VCAM-1 ELISA Kit

Vascular Cell Adhesion Molecule 1 ELISA Kit

Catalog No. Product Name Size List Price (US$) Quantity
EK000038-EK0537-1 Human VCAM-1 ELISA Kit 1 plate, 96T/plate 369.00
EK000038-EK0537-4 Human VCAM-1 ELISA Kit 4 plates, 96T/plate 1328.00
Description

EK000038-EK0537: Human VCAM-1 ELISA Kit

Range 156pg/ml-10,000pg/ml
Sensitivity < 10 pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitive detection of human VCAM-1 in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.

Background

Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.1 Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.2 Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.3 The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.1 The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE.

Principle

The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.

Reference

1. Cybulsky, M. I.; Fries, J. W. U.; Williams, A. J.; Sultan, P.; Eddy, R.; Byers, M.; Shows, T.; Gimbrone, M. A., Jr.; Collins, T. Gene structure, chromosomal location, and basis for alternative mRNA splicing of the human VCAM1 gene. Proc. Nat. Acad. Sci. 88: 7859-7863, 1991.
2. Besemer, J.; Harant, H.; Wang, S.; Oberhauser, B.; Marquardt, K.; Foster, C. A.; Schreiner, E. P.; de Vries, J. E.; Dascher-Nadel, C.; Lindley, I. J. D. Selective inhibition of cotranslational translocation of vascular cell adhesion molecule 1. (Letter) Nature 436: 290-293, 2005.
3. Garmy-Susini, B.; Jin, H.; Zhu, Y.; Sung, R.-J.; Hwang, R.; Varner, J. Integrin alpha-4-beta-1--VCAM-1--mediated adhesion between endothelial and mural cells is required for blood vessel maturation. J. Clin. Invest. 115: 1542-1551, 2005.

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