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EK000048-EK0641: Human Survivin ELISA Kit
Range 62.5pg/ml-4000pg/ml
Sensitivity < 2 pg/ml
Specificity; no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human Survivin in sera, plasma, body fluids,
tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
Survivin (also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis.1 Survivin is prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast in vivo. It is also found in approximately 50% of high-grade non-Hodgkin's lymphomas.2 Survivin encodes a deduced 142-amino acid protein and localizes on chromosome 17q25. The overexpression of it in cancer may overcome an apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis.3 The standard product used in this kit is recombinant human Survivin with the molecular mass of 34 KDa.
Principle
The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.
Reference
1. Blanc-Brude, O. P.; Yu, J.; Simosa, H.; Conte, M. S.; Sessa, W. C.; Altieri, D. C. : Inhibitor of apoptosis protein survivin regulates vascular injury. Nature Med. 8: 987-994, 2002.
2. Ambrosini, G.; Adida, C.; Altieri, D. C. : A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nature Med. 3: 917-921, 1997.
3. Li, F.; Ambrosini, G.; Chu, E. Y.; Plescia, J.; Tognin, S.; Marchisio, P. C.; Altieri, D. C. : Control of apoptosis and
mitotic spindle checkpoint by survivin. Nature 396: 580-584, 1998.
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