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EK000141-EK0895: Human P53 ELISA Kit
Range 156pg/ml-10,000pg/ml
Sensitivity < 10pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human P53 in serum, plasma or cell.
Expiration: four months at 4°C and eight months at -20°C.
Background
P53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation.
Principle
The human P53 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human P53 specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human P53 amount of sample captured in plate.
Reference
1. Matlashewski G, Lamb P, Pim D, Peacock J, Crawford L, Benchimol S (December 1984). "Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene". EMBO J. 3 (13): 3257?2. PMC 557846. PMID 6396087.
2. Isobe M, Emanuel BS, Givol D, Oren M, Croce CM (1986). "Localization of gene for human p53 tumour antigen to band 17p13". Nature 320 (6057): 84?. doi:10.1038/320084a0. PMID 3456488.
3. Kern SE, Kinzler KW, Bruskin A, Jarosz D, Friedman P, Prives C, Vogelstein B (June 1991). "Identification of p53 as a sequence-specific DNA-binding protein". Science (journal) 252 (5013): 1708?1. doi:10.1126/science.2047879. PMID 2047879.
4. McBride OW, Merry D, Givol D (1986). "The gene for human p53 cellular tumor antigen is located on chromosome 17 short arm (17p13)". Proc. Natl. Acad. Sci. U.S.A. 83 (1): 13034. doi:10.1073/pnas.83.1.130. PMC 322805. PMID 3001719.
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