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Conditions of optimal recombinant Human NKG2D/CD314 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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BP005196: Human NKG2D/CD314 Protein with the N-hFc-Flag Tag
Source: The recombinant Human NKG2D/CD314 Protein, (amino acids Phe78-Val216) fused with a hFc-Flag tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human NKG2D/CD314 protein with the hFc-Flag tag at the N-terminus has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human MICA protein with the N-hFc-Flag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human NKG2D protein with the N-hFc tag with the EC50 of 0.81 ug/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human NKG2D/CD314 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP005399: Human NKG2D/CD314 Protein with the N-His-Avi Tag
Source: The recombinant Human NKG2D/CD314 Protein, (amino acids Phe78-Val216) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human NKG2D/CD314 protein with the His-Avi tag at the N-terminus has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-38 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human NKG2D protein with the N-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-NKG2D Antibody protein with the N-hFc tag with the EC50 of 6.6 ng/ml determined by ELISA (QC Test). The affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human NKG2D/CD314 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Human NKG2D/CD314 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following NKG2D Protein :
Biotinylated Human NKG2D Protein
Cynomolgus NKG2D Protein
FITC-Labeled Human NKG2D Protein
Human NKG2D Protein
Mouse NKG2D Protein
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