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Human IL-12 Protein

CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12

Catalog No. Product Name Size List Price (US$) Quantity
BP005622 Human IL-12 Protein with the C-His-Avi Tag 100UG 525.00
BP005622 Human IL-12 Protein with the C-His-Avi Tag 500UG 1750.00
Description

BP005622: Human IL-12 Protein with the C-His-Avi Tag

Source: The recombinant Human IL-12 Protein, (amino acids Ile23-Ser328(IL-12B) & Arg23-Ser219(IL-12A)) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human IL-12 protein with the His-Avi tag at the C-terminus has a predicted MW of 34.7 kDa(IL-12B) & 25.5 kDa(IL-12A). Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human IL-12 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-IL-12 Antibody protein with the C-hFc tag with the EC50 of 2.1 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human IL-12 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human IL-12 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following IL-12 Protein :
Biotinylated Human IL-12 Protein
Cynomolgus IL-12 Protein
Human IL-12 Protein
IL-12 Protein
Mouse IL-12 Protein
Human IL-12 R beta 1 Protein
Cynomolgus IL-12B Protein
Human IL-12B Protein

Syd Labs provides the following IL-12 ELISA Kit :
Human IL-12 ELISA Kit
Mouse IL-12 ELISA Kit

Syd Labs provides the following IL-12 Antibody :
IL-12 Antibody
IL-12B Antibody
IL-12p75 Antibody

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