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Human IL-1 alpha ELISA Kit

Interleukin-1α ELISA Kit
Catalog No. Product Name Size List Price (US$) Quantity
EK000018-EK0389-1 Human IL-1A ELISA Kit 1 plate, 96T/plate 312.00
EK000018-EK0389-4 Human IL-1A ELISA Kit 4 plates, 96T/plate 1061.00
Description

EK000018-EK0389: Human IL-1α ELISA Kit

Range 4.7pg/ml-300pg/ml
Sensitivity < 0.5pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human IL-1α in sera, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.

Background

Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair.1 IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases.2 Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2.3 Genetic polymorphisms at interleukin (IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis.4 The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis.5 The standard product used in this kit is recombinant human IL-1a with the molecular mass of 18kDa.

Principle

The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.

Reference

1. Lafage, M.; Maroc, N.; Dubreuil, P.; de Waal Malefijt, R.; Pebusque, M.-J.; Carcassonne, Y.; Mannoni, P. The human interleukin-1-alpha gene is located on the long arm of chromosome 2 at band q13. Blood 73: 104-107, 1989.
2. Bailly, S.; di Giovine, F. S.; Blakemore, A. I. F.; Duff, G. W. Genetic polymorphism of human interleukin-1-alpha. Europ. J. Immun. 23: 1240-1245, 1993.
3. Modi, W. S.; Masuda, A.; Yamada, M.; Oppenheim, J. J.; Matsushima, K.; O'Brien, S. J. Chromosomal localization of the human interleukin 1 alpha (IL-1-alpha) gene. Genomics 2: 310-314, 1988.
4. Diehl, S. R.; Wang, Y.; Brooks, C. N.; Burmeister, J. A.; Califano, J. V.; Wang, S.; Schenkein, H. A. Linkage disequilibrium of interleukin-1 genetic polymorphisms with early-onset periodontitis. J. Periodont. 70: 418-430, 1999.
5. Silver, A. R. J.; Masson, W. K.; George, A. M.; Adam, J.; Cox, R. The Il-1 alpha and beta genes are closely linked (less than 70 kb) on mouse chromosome 2. Somat. Cell Molec. Genet. 16: 549-556, 1990.

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