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Human HVEM Protein

CD270; CD40-like protein;- HVEM; LIGHTR; ATAR; TNFRSF14; TR2

Catalog No. Product Name Size List Price (US$) Quantity
BP005244 Human HVEM/TNFRSF14 Protein with the C-His Tag 100UG 255.00
BP005244 Human HVEM/TNFRSF14 Protein with the C-His Tag 500UG 850.00
BP005357 Human HVEM/TNFRSF14 Protein with the C-hFc Tag 100UG 270.00
BP005357 Human HVEM/TNFRSF14 Protein with the C-hFc Tag 500UG 900.00
Description

BP005244: Human HVEM/TNFRSF14 Protein with the C-His Tag

Source: The recombinant Human HVEM/TNFRSF14 Protein, (amino acids Leu39-Val202) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human HVEM/TNFRSF14 protein with the His tag at the C-terminus has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human HVEM protein with the C-His tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human CD160 protein with the C-hFc tag with the EC50 of 0.17 ug/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human HVEM/TNFRSF14 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005357: Human HVEM/TNFRSF14 Protein with the C-hFc Tag

Source: The recombinant Human HVEM/TNFRSF14 Protein, (amino acids Leu39-Val202) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human HVEM/TNFRSF14 protein with the hFc tag at the C-terminus has a predicted MW of 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human LIGHT protein with the C-hFc tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human HVEM protein with the C-hFc tag with the EC50 of 15.3 ng/ml determined by ELISA. The affinity constant of 1.44 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human HVEM/TNFRSF14 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human HVEM/TNFRSF14 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following HVEM Protein :
Human HVEM Protein

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