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Conditions of optimal recombinant Human Fc epsilon RI alpha/FCER1a Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP004330: Human Fc epsilon RI alpha/FCER1a Protein with the C-His Tag
Source: The recombinant Human Fc epsilon RI alpha/FCER1a Protein, (amino acids Val26-Gln205) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human Fc epsilon RI alpha/FCER1a protein with the His tag at the C-terminus has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Fc epsilon RI alpha protein with the C-His tag at 0.2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Fc epsilon RI alpha Antibody protein with the C-hFc tag with the EC50 of 4.0 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Fc epsilon RI alpha/FCER1a Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP004551: Human Fc epsilon RI alpha/FCER1a Protein with the C-hFc Tag
Source: The recombinant Human Fc epsilon RI alpha/FCER1a Protein, (amino acids Val26-Gln205) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human Fc epsilon RI alpha/FCER1a protein with the hFc tag at the C-terminus has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Fc epsilon RI alpha protein with the C-hFc tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Biotinylated Anti-Fc epsilon RI alpha Antibody protein with the C-hFc tag with the EC50 14.5 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Fc epsilon RI alpha/FCER1a Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Human Fc epsilon RI alpha/FCER1a Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following Fc Epsilon RI alpha Protein :
Human Fc epsilon RI alpha Protein
Mouse Fc Epsilon RI alpha Protein
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