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Conditions of optimal HSPA1B Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
The HSP70 family is found in many intracellular compartments such as chloroplasts, endoplasmic reticulum, mitochondria, and cytosol. These proteins are induced by a variation of biological stresses, including heat stress, in every organism. HSP70 has a range of functions. For example: HSP70 acts as molecular chaperones facilitating the assembly of multi-protein complexes, HSP70 take part in the translocation of polypeptides across cell membranes and to the nucleus, and HSP70 assists in the proper folding of nascent polypeptide chains.
HSP70 is a mitochondrial import equipment and has a crucial part in the cytosolic endoplasmic reticulum. Lately, extracellular localized HSP was discovered to take an important part in the induction of a cellular immune response.
BP002300-HSP-021: Recombinant Human Heat Shock 70 kDa protein 1B (HSPA1B)
Source: E. coli-derived.
Recombinant Human HSPA1B Protein is a single, non-glycosylated polypeptide chain containing 650 amino acids having a molecular mass of 71.16 kDa. Recombinant Human HSPA1B Protein is fused to a 10 amino acids His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Applications: Western blotting, ELISA.
Formulation: The protein was filtered (0.4 um) and lyophilized from a 0.5 mg/ml solution containing 50 mM phosphate buffer, 75 mM NaCl, pH 7.4.
Reconstitution: It is recommended to add deionized water to prepare a working stock solution of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
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