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Human Nectin-4 Protein

EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4

Catalog No. Product Name Size List Price (US$) Quantity
BP005497 Human Nectin-4 Protein with the C-hFc Tag 100UG 315.00
BP005497 Human Nectin-4 Protein with the C-hFc Tag 500UG 1050.00
BP005423 Human Nectin-4 Protein with the C-His-Avi Tag 100UG 270.00
BP005423 Human Nectin-4 Protein with the C-His-Avi Tag 500UG 900.00
Description

BP005497: Human Nectin-4 Protein with the C-hFc Tag

Source: The recombinant Human Nectin-4 Protein, (amino acids Gly32-Ser349) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human Nectin-4 protein with the hFc tag at the C-terminus has a predicted MW of 61.1 kDa. Due to glycosylation, the protein migrates to 72-74 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Nectin-4 protein with the C-hFc tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human Nectin-1 protein with the C-His tag with the EC50 of 2.1 ug/ml determined by ELISA (QC Test). The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Nectin-4 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005423: Human Nectin-4 Protein with the C-His-Avi Tag

Source: The recombinant Human Nectin-4 Protein, (amino acids Gly32-Val351) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human Nectin-4 protein with the His-Avi tag at the C-terminus has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human Nectin-4 protein with the C-His-Avi tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-Nectin-4 Antibody protein with the C-hFc tag with the EC50 of 8.0 ng/ml determined by ELISA (QC Test).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Nectin-4 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Human Nectin-4 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs provides the following Nectin-1 Protein :
Biotinylated Human Nectin-1 Protein
Human Nectin-1 Protein

Syd Labs provides the following Nectin-2 Protein :
Biotinylated Human Nectin-2 Protein
Human Nectin-2 Protein
Mouse Nectin-2 Protein

Syd Labs provides the following Nectin-3 Protein :
Biotinylated Human Nectin-3 Protein
Human Nectin-3 Protein

Syd Labs provides the following Nectin-4 Protein :
Biotinylated Human Nectin-4 Protein
Biotinylated Human Nectin-4 Protein IgV Domain
Cynomolgus Nectin-4 Protein
Human Nectin-4 Protein
Human Nectin-4 Protein IgV Domain
Mouse Nectin-4 Protein

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