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EK000066-EK0578: Human Growth Hormone ELISA Kit
Range 15.6pg/ml-1000pg/ml
Sensitivity < 2 pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitive detection of human Growth Hormone in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone (GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency (GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22KDa.
Principle
The ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.
Reference
1. Baum, H. B. A.; Katznelson, L.; Sherman, J. C.; Biller, B. M. K.; Hayden, D. L.; Schoenfeld, D. A.; Cannistraro, K. E.; Klibanski, A. : Effects of physiological growth hormone (GH) therapy on cognition and quality of life in patients with adult-onset GH deficiency. J. Clin. Endocr. Metab. 83: 3184-3189, 1998
2. Niall, H. D.; Hogan, M. L.; Sauer, R.; Rosenblum, I. Y.; Greenwood, F. C. : Sequence of pituitary and placental lactogenic and growth hormones: evolution from a primordial peptide by gene reduplication. Proc. Nat. Acad. Sci. 68: 866-869, 1971.
3. Owerbach, D.; Rutter, W. J.; Martial, J. A.; Baxter, J. D.; Shows, T. B. : Genes for growth hormone, chorionic somatomammotropin and growth hormone-like genes on chromosome 17 in humans. Science 209: 289-292, 1980.
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