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Conditions of optimal GDNF Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.
GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
BP001001-CYT-305: Recombinant Human Glial Cell Line-Derived Neurotrophic Factor (GDNF) Protein
Source: E. coli-derived.
N-terminal sequence analysis: Met-Ser-Pro-Asp-Lys.
A homodimer, non-glycosylated, polypeptide chain containing 2 x 135 amino acids and having a total molecular mass of 30,360 Dalton.
The ED50, calculated by the dose-dependant dopamin uptake in rat mesencephalic cultures was found to be 5-10 ng/ml, corresponding to a specific activity of 100,000-200,000 Units/mg
Purity: > 98% by SDS-PAGE and RP-HPLC.
Formulation: GDNF was lyophilized after dialysis against 10 mM sodium citrate and 150 mM NaCl.
Reconstitution: It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18 M?-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other GDNF Proteins:
Recombinant Human GDNF Protein
GDNF Antibodies:
GDNF Polyclonal Antibody
GDNF ELISA Kits:
Human GDNF ELISA Kit
Rat GDNF ELISA Kit
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