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Conditions of optimal G6PD Enzyme performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
G6PD is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. The NADPH maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency causes acute hemolytic anemia, neonatal jaundice or acute hemolysis. G6PD is a cytosolic enzyme encoded by an X-linked gene whose main function is to produce NADPH, a crucial electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD produces pentose sugars for nucleic acid synthesis and is a main producer of NADPH reducing power.
BP000565-ENZ-608: Recombinant Human G6PD (Glucose-6-phosphate 1-dehydrogenase)
Source: Hi-5 cells-derived.
A single polypeptide chain containing 535 amino acids (1-515) and having a molecular mass of 61.4 kDa. G6PD is fused to a 20 amino acid His-tag at N-terminus.
Specific activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
Purity > 95%, by SDS-PAGE.
Formulation: The G6PD solution (0.5 mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 0.1 mM PMSF, 2 mM EDTA, 2 mM DTT, 200 mM NaCl and 20% glycerol.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
Other G6PD Enzymes:
Recombinant Human G6PD Enzyme
Recombinant E. coli G6PD Enzyme
G6PD Antibodies:
G6PD Monoclonal Antibody
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