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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal FVIIa Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Coagulation factor VII is a vitamin K-dependent factor which is essential for hemostasis. It circulates in the blood as a zymogen which is later converted to an active form by factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of factor VII, a heavy chain with a catalytic domain and a light chain with 2 EGF-like domains are generated, and the two chains are held together by a disulfide bond. The presence of factor III and calcium ions further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Alternative splicing of factor VII results in 2 transcripts. Defects in coagulation factor VII can cause coagulopathy. Coagulation factor VII initiates the extrinsic pathway of blood coagulation. Minor proteolysis converts factor VII to factor VIIa by factors Xa, XIIa, IXa, or thrombin. Factor VIIa also converts factor IX to factor IXa in the presence of tissue factor and calcium.
BP001902-PRO-331: Recombinant Human Coagulation Factor VIIa (FVIIa) Protein
Source: BHK cells-derived.
Recombinant Human Factor VIIa is a glycosylated polypeptide two-chain dimer consisting of 406 amino acids with a molecular weight of 50 kD. The Factor-VIIa is purified by proprietary chromatographic techniques.
The potency per mg of recombinant human FVIIa protein was tested and found to be 50,000 Units/mg.
Purity: > 98.0% as determined by (a) Analysis by RP-HPLC; (b) Analysis by SDS-PAGE.
Formulation: The protein 1 mg/ml was lyophilized after from a sterile solution containing 4.86 mg sodium chloride, 2.45 mg calcium chloride dihydrate, 50 mg mannitol and 116 ?g Tween 80.
Reconstitution: It is recommended to reconstitute the lyophilized Factor-VIIa in sterile 18 M?-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
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