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Conditions of optimal FGF-1 / aFGF / FGF1 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
FGF acidic, also known as aFGF/FGF-1/FGF1, ECGF, and HBGF 1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic protein (aFGF/FGF-1/FGF1) is a potent growth factor for fibroblasts and endothelial cells (1). FGF acidic protein (aFGF/FGF-1/FGF1) is involved in wound repair, angiogenesis, and development (1). FGF acidic protein (aFGF/FGF-1/FGF1) is secreted from cells via an endoplasmic reticulum/Golgi independent mechanism (1,2). The ability of FGF acidic protein (aFGF/FGF-1/FGF1) to bind to heparin sulfate is required for its ability to interact with FGF receptors and induce signaling (1-4). There are four distinct FGF receptors and each has multiple splice variants (1,3). FGF acidic protein (aFGF/FGF-1/FGF1) binds with high affinity to many, but not all, FGFRs (1). Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLC /PKC, and PI3K/Akt pathways (1).
BP000029-C043: Recombinant Mouse Fibroblast Growth Factor 1 (FGF-1) Protein
Source: E. coli-derived.
Recombinant Mouse Fibroblast Growth Factor 1 (FGF-1) Protein is expressed with sequence (F16-D155) of Mouse FGF-A.
N-terminal sequence analysis: Phe-Asn-Leu-Pro-Leu.
The specific activity of Recombinant Mouse Fibroblast Growth Factor 1 (FGF-1) Protein is calculated by its ability to 2.0 x 106 IU/mg. The ED50 for this effect is typically less than 0.5 ug/ml as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of Heparin.
Purity: >95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).
Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM Tris-HCl, 500 mM NaCl, pH 6.6.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping: The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other FGF-1 / aFGF / FGF1 Proteins:
Recombinant Human FGF Acidic 136 (aFGF)
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