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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Conditions of optimal FGF-1 / aFGF / FGF1 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
FGF acidic, also known as aFGF/FGF-1/FGF1, ECGF, and HBGF 1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic protein (aFGF/FGF-1/FGF1) is a potent growth factor for fibroblasts and endothelial cells (1). FGF acidic protein (aFGF/FGF-1/FGF1) is involved in wound repair, angiogenesis, and development (1). FGF acidic protein (aFGF/FGF-1/FGF1) is secreted from cells via an endoplasmic reticulum/Golgi independent mechanism (1,2). The ability of FGF acidic protein (aFGF/FGF-1/FGF1) to bind to heparin sulfate is required for its ability to interact with FGF receptors and induce signaling (1-4). There are four distinct FGF receptors and each has multiple splice variants (1,3). FGF acidic protein (aFGF/FGF-1/FGF1) binds with high affinity to many, but not all, FGFRs (1). Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLC /PKC, and PI3K/Akt pathways (1).
BP000029-GD3: Recombinant Human FGF Acidic 136 (aa 19-155) Protein
Source: E. coli-derived.
N-terminal sequence analysis: Gly-Leu18-Asp155, with an N-terminal Gly
Predicted molecular mass: 15.5 kDa
The FGF acidic protein (aFGF/FGF-1/FGF1) activity is measured in a NIH/3T3 cell proliferation assay. The ED50 for this effect is typically 0.1-0.3 ng/ml in the presence of 10 ug/ml of heparin.
Endotoxin: <1.0 EU per 1 ug of the protein by the LAL method.
Purity: > 95% by SDS-PAGE under reducing conditions and visualized by silver stain.
Formulation: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.2.
Reconstitution: Reconstitute at 10 mg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping: The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
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