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Conditions of optimal EIF4A3 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Eukaryotic initiation factor 4A-III (EIF4A3) is a member of the DEAD box helicase family and eIF4A subfamily. DEAD box proteins, distinguished by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. These proteins are involved in several cellular processes including alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. EIF4A3 is a part of a splicing-dependent multiprotein exon junction complex (EJC) accumulated at splice junction on mRNAs. Based upon their distribution patterns, some members of the DEAD box helicase family are thought to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
BP001962-PRO-1007: Recombinant Human Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) Protein
Source: E. coli-derived.
Recombinant Human EIF4A3 protein is a single, non-glycosylated polypeptide chain containing 435 amino acids (1-411 a.a.) and having a molecular mass of 49.4 kDa. Recombinant Human EIF4A3 protein is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Purity: > 95.0% as determined by SDS-PAGE.
Formulation: EIF4A3 protein solution (0.5 mg/ml) containing 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 30% glycerol and 200 mM NaCl.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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