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Conditions of optimal EIF3J Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
Eukaryotic translation initiation factor 3 subunit J (EIF3J) is a member of the EIF-3 subunit J family. EIF3 has a crucial role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to produce the 40S initiation complex. EIF3J binds to the aminoacyl (A) site and mRNA access channel of the 40S subunit, setting EIF3J directly in the ribosomal decoding center. In addition, EIF3J interacts with eIF1A and reduces 40S subunit affinity for mRNA. An elevated affinity for mRNA is restored upon enlistment of initiator tRNA, despite the fact EIF3J remains in the mRNA-binding cleft in the presence of tRNA.
BP001960-PRO-970: Recombinant Human Eukaryotic Translation Initiation Factor 3J (EIF3J) Protein
Source: E. coli-derived.
Recombinant Human EIF3J protein is a single, non-glycosylated polypeptide chain containing 210 amino acids (70-258) and having a molecular mass of 24 kDa (Molecular weight on SDS-PAGE will appear higher). Recombinant Human EIF3J protein is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Purity: > 90.0% as determined by SDS-PAGE.
Formulation: The EIF3J solution (0.5 mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 10% glycerol and 200 mM NaCl.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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