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EG-VEGF ELISA Kit

Endocrine Gland derived Vascular Endothelial Growth Factor, PROKR1
Catalog No. Product Name Size List Price (US$) Quantity
EK000142-EK0575-1 Human EG-VEGF ELISA Kit 1 plate, 96T/plate 480.00
EK000142-EK0575-4 Human EG-VEGF ELISA Kit 4 plates, 96T/plate 1632.00
Description

EK000142-EK0575: Human EG-VEGF ELISA Kit

Range 15.6pg/ml-1000pg/ml
Sensitivity < 1pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitative detection of human EG-VEGF in serum, plasma, or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.

Background

EG-VEGF, Endocrine gland derived vascular endothelial growth factor, is a human protein encoded by by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. The standards of this kit are recombinant human EG-VEGF (A20-F105), with molecular weight of 10.5 KDa.

Principle

The human EG-VEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human EG-VEGF specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EG-VEGF amount of sample captured in plate.

Reference

1. LeCouter, J., Kowalski, J., Foster, J., Hass, P., Zhang, Z., Dillard-Telm, L., Frantz, G., Rangell, L., DeGuzman, L., Keller, G.-A., Peale, F., Gurney, P., Hillan, K. J., Ferrara, N. Identification of an angiogenic mitogen selective for endocrine gland endothelium. Nature 412: 877-884, 2001. [PubMed: 11528470].
2. Li, M., Bullock, C. M., Knauer, D. J., Ehlert, F. J., Zhou, Q. Y. Identification of two prokineticin cDNAs: recombinant proteins potently contract gastrointestinal smooth muscle. Molec. Pharm. 59: 692-698, 2001. [PubMed: 11259612]

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