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EK000073-EK0561: Human E-Cadherin ELISA Kit
Range 156pg/ml-10,000pg/ml
Sensitivity < 15pg/ml
Specificity: no detectable cross-reactivity with any other cytokine.
Application: for quantitive detection of human E-Cadherin in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.
Background
E-Cadherin, also called Cadherin 1 (CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2.1 E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas.2 E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems.3 The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.
Principle
The E-Cadherin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. The target specific antibodies are precoated onto 96-well plates. The target from the sample is bound to the microwell. The biotinylated target specific detection antibodies are added to the microwells and followed by washing with the PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with the PBS or TBS buffer. TMB, the HRP (horseradish peroxidase) substrate, is used to visualize color change resulting from the HRP enzymatic reaction. TMB is catalyzed by HRP to produce the blue color. The color changes into yellow after the acidic stop solution is added. The density of the yellow color is proportional to the target amount from the sample captured in the microwells.
Reference
1. Berx, G.; Staes, K.; van Hengel, J.; Molemans, F.; Bussemakers, M. J. G.; van Bokhoven, A.; van Roy, F. : Cloning and characterization of the human invasion suppressor gene E-cadherin (CDH1). Genomics 26: 281-289, 1995.
2. Becker, K.-F.; Atkinson, M. J.; Reich, U.; Becker, I.; Nekarda, H.; Siewert, J. R.; Hoefler, H. : E-Cadherin gene mutations provide clues to diffuse type gastric carcinomas. Cancer Res. 54: 3845-3852, 1994.
3. Batlle, E.; Sancho, E.; Franci, C.; Dominguez, D.; Monfar, M.; Baulida, J.; Garcia de Herreros, A. : The transition factor Snail is a repressor of E-cadherin gene expression in epithelial tumour cells. Nature Cell Biol. 2: 84-89, 2000.
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