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Conditions of optimal DDIT4 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
DDIT4 has a role in the regulation of reactive oxygen species. DDIT4 is upregulated at the transcriptional level as a reaction to stress due to DNA damage and glucocorticoid treatment. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase frequently referred to as mTOR. DDIT4 is vital in the coupling of extra- and intracellular cues to mTOR regulation.
BP001939-PRO-094: Recombinant Human DNA Damage Inducible Transcript 4 (DDIT4) Protein
Source: E. coli-derived.
Recombinant Human DDIT4 protein is a single, non-glycosylated polypeptide chain containing 252 amino acids (1-232.a.a) and having a molecular mass of 27.5 kDa. Recombinant Human DDIT4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Purity: > 90% as determined by SDS-PAGE.
Formulation: The DDIT4 protein solution (0.25 mg/ml) is formulated in 20 mM Tris-HCl buffer (pH8.0), 0.2 M NaCl, 5 mM DTT, 1 mM EDTA and 30% glycerol.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
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