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Conditions of optimal recombinant Cynomolgus PCSK9 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP003875: Cynomolgus PCSK9 Protein with the C-His Tag
Source: The recombinant Cynomolgus PCSK9 Protein, (amino acids Glu151-Gln811) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:Due to autocatalytic cleavage, The recombinant Cynomolgus PCSK9 Protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-70 kDa (pro-form) and 15-20 kDa (mature form) based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Cynomolgus PCSK9 protein with the C-His tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve Anti-PCSK9 Antibody protein with the C-hFc tag with the EC50 of 55.7 ng/ml determined by ELISA (QC Test). The affinity constant of 0.27 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Cynomolgus PCSK9 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Shipping: The recombinant Cynomolgus PCSK9 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.
Syd Labs provides the following PCSK9 Protein :
Cynomolgus PCSK9 Protein
Human PCSK9 Protein
Mouse PCSK9 Protein
PCSK9 Protein
Syd Labs provides the following PCSK9 Antibody :
Evolocumab Biosimilar, PCSK9 Monoclonal Antibody
PCSK9 Antibody
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