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Conditions of optimal CD14 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
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Introduction
CD14 is a cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS binding protein (LBP). Toll like receptors have also been implicated in the transduction of CD14-LPS signals. Soluble CD14 can be released from the cell surface by phosphatidyinositolspecific phospholipase C and has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.
BP000308-PRO-536: Recombinant Human Monocyte Differentiation Antigen CD14 (CD14) Protein, CHO
Source: CHO-cells-derived.
Predicted molecular mass: 50 kDa.
Biological Activity: Up to 20 ug/ml CD14 inhibit binding of FITC-LPS (0.5 ug/ml) to 6 x 105 CD14+CHO transfectants (FACS).
Purity: > 95% by SDS-PAGE.
Formulation: Lyophilized from a concentrated protein solution (1.0 ug/ml) containing phosphate-buffered saline, pH 7.2.
Reconstitution: It is recommended to reconstitute the lyophilized CD14 in sterile 18 M?-cm H2O not less than 100 ug/ml. Further dilutions should be made with phosphate buffered saline (PBS).
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other CD14 Proteins:
Recombinant Mouse CD14 Protein
Recombinant Human CD14 Protein
CD14 Antibody:
FITC Conjugated CD14 Monoclonal Antibody
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