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Conditions of optimal MCP 2 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
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Introduction
Chemokine (C-C motif) ligand 8 (CCL8) is a small cytokine belonging to the CC chemokine family that was once called monocyte chemotactic protein-2 (MCP-2). The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. MCP-2 is chemotactic for and activates a many different immune cells, including mast cells, eosinophils and basophils, (that are implicated in allergic responses), and monocytes, T cells, and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to several different cell surface receptors called chemokine receptors. These receptors include CCR1, CCR2B and CCR5.
BP000276-CHM-316: Recombinant Human C-C Motif Chemokine 8 (CCL8) Protein
Source: E. coli-derived.
N-terminal sequence analysis: Gln-Pro-Asp-Ser-Val.
A non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8904 Dalton.
The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human THP-1 cells. Significant calcium mobilization is observed with 500 ug/ml of recombinant human MCP-2. Human MCP-2 also induces dose dependent chemotaxis of human THP-1 cells with an ED50=30-100 ug/ml corresponding to a Specific Activity of 10,000-33,334 IU/mg.
Purity: > 95% by SDS-PAGE and RP-HPLC.
Formulation: Lyophilized from a concentrated (1 ug/ml) sterile solution containing no additives.
Reconstitution: It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-2in sterile 18 M?-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other CCL8 Proteins:
Recombinant Mouse CCL8 Protein
Recombinant Human CCL8 Protein
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