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Conditions of optimal β-NGF Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
Beta-Nerve Growth Factor (β-NGF) protein, a small secreted protein, is important for the growth, maintenance, and survival of sympathetic and sensory neurons (nerve cells). beta-NGF, Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin-3 (NT-3), and Neurotrophin 4/5 (NT-4/5) belong to the neurotrophin family of nerve growth factors. Mutations in the encoding gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of the protein expression is associated with allergic rhinitis.
BP000997-GD23: Recombinant Human Beta-Nerve Growth Factor (β-NGF) Protein
Source: E. coli-derived.
Recombinant Human Beta-Nerve Growth Factor (β-NGF) Protein is a non-glycosylated non-covalent homodimer of two 118 amino acid polypeptides each with a molecular mass of 13.4 kDa.
N-terminal sequence analysis: Ser-Ser-Ser-His-Pro.
The ED50 of the recombinant human beta-Nerve Growth Factor (β-NGF) protein as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells was < 1.0 ng/ml, corresponding to a specific activity of > 1 x 10^6 IU/mg.
Endotoxin: < 0.1 ng/ug (1 EU/ug).
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 250 mM NaCl, pH 7.0.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Other beta-NGF Proteins:
Recombinant human beta-NGF protein produced in HEK
Recombinant human beta-NGF protein produced in CHO
Recombinant human pro-NGF protein
Mouse beta-NGF protein
Recombinant mouse beta-NGF protein
beta-NGF Antibodies:
beta-NGF polyclonal antibody
beta-NGF polyclonal antibody
beta-NGF ELISA Kits:
Human beta-NGF ELISA kit
Mouse beta-NGF ELISA kit
Rat beta-NGF ELISA kit
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