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Conditions of optimal BCL2 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
BP001830-PRO-630: Recombinant Human B-Cell Leukemia/Lymphoma 2 (BCL2) Protein
Source: E. coli-derived.
Recombinant Human Bcl-2 is a single, non-glycosylated polypeptide chain containing amino acids 1-206. The wild type Bcl-2 is missing 12 amino acids from C-terminus. C-terminus is fused to His-Tag. C-terminus his-tag, mimics the deleted C-terminus membrane domain thus maintaining its biological activity. Bcl-2 is purified by proprietary chromatographic techniques.
Purity: > 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Applications: Input marker or positive control (Western Blotting), Function study.
Formulation: The protein contains 10 mM Tris-HCl pH-8, 1 mM EDTA and 250 mM NaCl.
Reconstitution: Suspend Bcl-2 in 100 ul of 0.5 M Acetic acid, over night at 4?. Dilute 10 fold into selected buffer system. BCL-2 has tendency to form intramolecular disulfide bond, 5 mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10 mM DTT is recommended.
Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
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